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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
26/09/2014 |
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Actualizado : |
18/03/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
IBÁÑEZ, F.; REZANO, A.; FREDES, A.; RODRIGUEZ, G.; REGGIO, A.; VILARÓ, F. |
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Afiliación : |
FACUNDO IBÁÑEZ SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANABELA REZANO MATKOVICH, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ALEJANDRO DAVID FREDES SIVOPLAS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; GUSTAVO ADOLFO RODRIGUEZ TEXEIRA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ADRIANA MARIA REGGIO BIGANZOLI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; FRANCISCO LUIS VILARO PAREJA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Development of a rapid microplate assay for determining pungency in onion breeding program. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Acta Horticulturae (ISHS), 2012, no. 935, p. 61-65. |
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ISSN : |
0567-7572 |
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DOI : |
10.17660/ActaHortic.2012.935.8 |
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Idioma : |
Inglés |
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Notas : |
XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding. |
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Contenido : |
ABSTRACT.
Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95). |
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Thesagro : |
AJO. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02138naa a2200229 a 4500
001 1050611
005 2022-03-18
008 2012 bl uuuu u00u1 u #d
022 $a0567-7572
024 7 $a10.17660/ActaHortic.2012.935.8$2DOI
100 1 $aIBÁÑEZ, F.
245 $aDevelopment of a rapid microplate assay for determining pungency in onion breeding program.$h[electronic resource]
260 $c2012
500 $aXXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding.
520 $aABSTRACT. Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95).
650 $aAJO
700 1 $aREZANO, A.
700 1 $aFREDES, A.
700 1 $aRODRIGUEZ, G.
700 1 $aREGGIO, A.
700 1 $aVILARÓ, F.
773 $tActa Horticulturae (ISHS), 2012, no. 935, p. 61-65.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
08/09/2014 |
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Actualizado : |
06/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 1 |
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Autor : |
UMPIÉRREZ-FALAICHE, M.; GARMENDIA, G.; PEREYRA, S.; RODRÍGUEZ-HARALAMBIDES, A.; WARD, T.J.; VERO, S. |
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Afiliación : |
SILVIA ANTONIA PEREYRA CORREA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Regional differences in species composition and toxigenic potential among Fusarium head blight isolates from Uruguay indicate a risk of nivalenol contamination in new wheat production areas. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
International Journal of Food Microbiology, v. 166, n. 1, p. 135-140, 2013 |
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ISSN : |
0168-1605 |
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DOI : |
10.1016/j.ijfoodmicro.2013.06.029 |
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Idioma : |
Inglés |
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Notas : |
Article history:Received 15 April 2013/Received in revised form 18 June 2013/Accepted 23 June 2013/Available online 1 July 2013. |
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Contenido : |
Abstract
Members of the Fusarium graminearum species complex (FGSC) are the primary cause of Fusarium head blight (FHB) of wheat, and frequently contaminate grain with trichothecene mycotoxins that pose a serious threat to food safety and animal health. The species identity and trichothecene toxin potential of 151 FGSC isolates collected from wheat in Uruguay were determined via multilocus genotyping. Although F. graminearum with the 15ADON trichothecene type accounted for 86% of the isolates examined, five different FGSC species and all three trichothecene types were identified in this collection. This is the first report of Fusarium asiaticum, Fusarium brasilicum, Fusarium cortaderiae, and Fusarium austroamericanum from Uruguay. In addition, we observed significant (P<0.001) regional differences in the composition of FGSC species and trichothecene types within Uruguay. Isolates of F. graminearum with the 15ADON type were the most prevalent in western provinces (95%), while F. asiaticum (43%) and the NIV type (61%) predominated in the new wheat production zone in Cerro Largo along Uruguay's eastern border with Brazil. F. graminearum isolates (15ADON type) were significantly (P<0.005) more aggressive on wheat than were isolates from the other species examined (NIV or 3ADON types). However, F. graminearum isolates (15ADON type) were significantly (P<0.05) more sensitive to tebuconazole than isolates from other species (NIV type). These results document substantial heterogeneity among the pathogens responsible for FHB in Uruguay. In addition, the regional predominance of the NIV trichothecene type is of significant concern to food safety and indicates that additional monitoring of nivalenol levels in grain may be required. MenosAbstract
Members of the Fusarium graminearum species complex (FGSC) are the primary cause of Fusarium head blight (FHB) of wheat, and frequently contaminate grain with trichothecene mycotoxins that pose a serious threat to food safety and animal health. The species identity and trichothecene toxin potential of 151 FGSC isolates collected from wheat in Uruguay were determined via multilocus genotyping. Although F. graminearum with the 15ADON trichothecene type accounted for 86% of the isolates examined, five different FGSC species and all three trichothecene types were identified in this collection. This is the first report of Fusarium asiaticum, Fusarium brasilicum, Fusarium cortaderiae, and Fusarium austroamericanum from Uruguay. In addition, we observed significant (P<0.001) regional differences in the composition of FGSC species and trichothecene types within Uruguay. Isolates of F. graminearum with the 15ADON type were the most prevalent in western provinces (95%), while F. asiaticum (43%) and the NIV type (61%) predominated in the new wheat production zone in Cerro Largo along Uruguay's eastern border with Brazil. F. graminearum isolates (15ADON type) were significantly (P<0.005) more aggressive on wheat than were isolates from the other species examined (NIV or 3ADON types). However, F. graminearum isolates (15ADON type) were significantly (P<0.05) more sensitive to tebuconazole than isolates from other species (NIV type). These results document substantial heterogenei... Presentar Todo |
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Palabras claves : |
AGRESSIVENESS; FGSC; FHB; FUSARIUM ASIATICUM; FUSARIUM AUSTROAMERICANUM; FUSARIUM BRASILICUM; FUSARIUM CORTADERIAE; FUSARIUM GRAMINEARUM SPECIES COMPLEX; FUSARIUM HEAD BLIGHT; MULTILOCUS GENOTYPING; NIVALENOL; TEBUCONAZOLE; TRICHOTHECENE MYCOTOXINS. |
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Thesagro : |
FITOPATOLOGIA; TRIGO; URUGUAY. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 03172naa a2200409 a 4500
001 1050037
005 2019-11-06
008 2013 bl uuuu u00u1 u #d
022 $a0168-1605
024 7 $a10.1016/j.ijfoodmicro.2013.06.029$2DOI
100 1 $aUMPIÉRREZ-FALAICHE, M.
245 $aRegional differences in species composition and toxigenic potential among Fusarium head blight isolates from Uruguay indicate a risk of nivalenol contamination in new wheat production areas.$h[electronic resource]
260 $c2013
500 $aArticle history:Received 15 April 2013/Received in revised form 18 June 2013/Accepted 23 June 2013/Available online 1 July 2013.
520 $aAbstract Members of the Fusarium graminearum species complex (FGSC) are the primary cause of Fusarium head blight (FHB) of wheat, and frequently contaminate grain with trichothecene mycotoxins that pose a serious threat to food safety and animal health. The species identity and trichothecene toxin potential of 151 FGSC isolates collected from wheat in Uruguay were determined via multilocus genotyping. Although F. graminearum with the 15ADON trichothecene type accounted for 86% of the isolates examined, five different FGSC species and all three trichothecene types were identified in this collection. This is the first report of Fusarium asiaticum, Fusarium brasilicum, Fusarium cortaderiae, and Fusarium austroamericanum from Uruguay. In addition, we observed significant (P<0.001) regional differences in the composition of FGSC species and trichothecene types within Uruguay. Isolates of F. graminearum with the 15ADON type were the most prevalent in western provinces (95%), while F. asiaticum (43%) and the NIV type (61%) predominated in the new wheat production zone in Cerro Largo along Uruguay's eastern border with Brazil. F. graminearum isolates (15ADON type) were significantly (P<0.005) more aggressive on wheat than were isolates from the other species examined (NIV or 3ADON types). However, F. graminearum isolates (15ADON type) were significantly (P<0.05) more sensitive to tebuconazole than isolates from other species (NIV type). These results document substantial heterogeneity among the pathogens responsible for FHB in Uruguay. In addition, the regional predominance of the NIV trichothecene type is of significant concern to food safety and indicates that additional monitoring of nivalenol levels in grain may be required.
650 $aFITOPATOLOGIA
650 $aTRIGO
650 $aURUGUAY
653 $aAGRESSIVENESS
653 $aFGSC
653 $aFHB
653 $aFUSARIUM ASIATICUM
653 $aFUSARIUM AUSTROAMERICANUM
653 $aFUSARIUM BRASILICUM
653 $aFUSARIUM CORTADERIAE
653 $aFUSARIUM GRAMINEARUM SPECIES COMPLEX
653 $aFUSARIUM HEAD BLIGHT
653 $aMULTILOCUS GENOTYPING
653 $aNIVALENOL
653 $aTEBUCONAZOLE
653 $aTRICHOTHECENE MYCOTOXINS
700 1 $aGARMENDIA, G.
700 1 $aPEREYRA, S.
700 1 $aRODRÍGUEZ-HARALAMBIDES, A.
700 1 $aWARD, T.J.
700 1 $aVERO, S.
773 $tInternational Journal of Food Microbiology$gv. 166, n. 1, p. 135-140, 2013
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